high capacity reverse transcriptase kit (Bio-Rad)
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Bio-Rad
high capacity reverse transcriptase kit
High Capacity Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+reverse+transcriptase/iScript+cDNA+Synthesis+Kit/pmc13654763-216-9-17
Average 99 stars, based on 48562 article reviews
High Capacity Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+reverse+transcriptase/iScript+cDNA+Synthesis+Kit/pmc13654763-216-9-17
Average 99 stars, based on 48562 article reviews
high capacity reverse transcriptase kit - by Bioz Stars,
2026-10
99/100 stars
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other:Article Title: Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cells Article Snippet: RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Scientific). Article Title: Lipopolysaccharide induces HIF-1α accumulation via MAPK p38-mediated mRNA stabilization and dexamethasone-sensitive protein stabilization. Article Snippet: RT-qPCR RNA was isolated using Norgen Total RNA Purification Plus kits (Geneflow, P4-0016) according to the manufacturer’s instructions. Article Title: Lipopolysaccharide induces HIF-1α accumulation via MAPK p38–mediated mRNA stabilization and dexamethasone-sensitive protein stabilization Article Snippet: Blots were imaged using Synthesized:Article Title: Hypoxia Affects Stem Cell Fate in Patient-Derived Ileum Enteroids in a HIF-1α-Dependent Manner Article Snippet: To determine the mRNA expression of genes using qRT-PCR, RNA was isolated from enteroids using the RNAeasy RNA extraction kit (Qiagen, Hilden, Germany #74136) according to the manufacturer’s instructions. .. Using 250 ng of total RNA, cDNA was synthesized using Article Title: Hypoxia Affects Stem Cell Fate in Patient-Derived Ileum Enteroids in a HIF-1α-Dependent Manner Article Snippet: To determine the mRNA expression of genes using qRT-PCR, RNA was isolated from enteroids using the RNAeasy RNA extraction kit (Qiagen, Hilden, Germany #74136) according to the manufacturer’s instructions. .. Using 250 ng of total RNA, cDNA was synthesized using Reverse Transcription:Article Title: Hypoxia Affects Stem Cell Fate in Patient-Derived Ileum Enteroids in a HIF-1α-Dependent Manner Article Snippet: To determine the mRNA expression of genes using qRT-PCR, RNA was isolated from enteroids using the RNAeasy RNA extraction kit (Qiagen, Hilden, Germany #74136) according to the manufacturer’s instructions. .. Using 250 ng of total RNA, cDNA was synthesized using Article Title: Nutrient Availability Dictates Cancer Metabolism–Based Therapeutic Responses to Nononcology Drugs Article Snippet: Cells were harvested for RNA extraction using the RNeasy Plus Mini kit (Qiagen, 74136). .. RNA samples were reverse transcribed to cDNA using Article Title: Impaired cortical development and translational control in a missense mouse model of DDX3X syndrome. Article Snippet: Cells were sorted into TRIzol LS Reagent (Invitrogen, 10296028), and RNA was extracted per the manufacturer’s protocol using either TRIzol LS reagent or the Zymo Directzol RNA Miniprep kit (Zymo Research, R2050). .. HEK-293T immortalized cells (ATCC, CRL-3216) were maintained at 37°C, 5% CO2 in DMEM high-glucose medium (Gibco, 11965092) supplemented with 10% fetal bovine serum (HyClone, SH30088.03HI) and 1% Pen-Strep (Gibco, 15140122). qRT-PCR analysis qRT-PCR analyses of gene expressionwere performed as previously described (Hoye et al., 2022), using Article Title: Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cells. Article Snippet: RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Scientific). .. For cDNA synthesis, 250 ng of total RNA was reverse-transcribed in a 20 μL reaction using 4 μL 5x iScript Reaction Mix and 1 μL Article Title: Hypoxia Affects Stem Cell Fate in Patient-Derived Ileum Enteroids in a HIF-1α-Dependent Manner Article Snippet: To determine the mRNA expression of genes using qRT-PCR, RNA was isolated from enteroids using the RNAeasy RNA extraction kit (Qiagen, Hilden, Germany #74136) according to the manufacturer’s instructions. .. Using 250 ng of total RNA, cDNA was synthesized using Quantitative RT-PCR:Article Title: Nutrient Availability Dictates Cancer Metabolism–Based Therapeutic Responses to Nononcology Drugs Article Snippet: Cells were harvested for RNA extraction using the RNeasy Plus Mini kit (Qiagen, 74136). .. RNA samples were reverse transcribed to cDNA using Article Title: Impaired cortical development and translational control in a missense mouse model of DDX3X syndrome. Article Snippet: Cells were sorted into TRIzol LS Reagent (Invitrogen, 10296028), and RNA was extracted per the manufacturer’s protocol using either TRIzol LS reagent or the Zymo Directzol RNA Miniprep kit (Zymo Research, R2050). .. HEK-293T immortalized cells (ATCC, CRL-3216) were maintained at 37°C, 5% CO2 in DMEM high-glucose medium (Gibco, 11965092) supplemented with 10% fetal bovine serum (HyClone, SH30088.03HI) and 1% Pen-Strep (Gibco, 15140122). qRT-PCR analysis qRT-PCR analyses of gene expressionwere performed as previously described (Hoye et al., 2022), using Real-time Polymerase Chain Reaction:Article Title: Nutrient Availability Dictates Cancer Metabolism–Based Therapeutic Responses to Nononcology Drugs Article Snippet: Cells were harvested for RNA extraction using the RNeasy Plus Mini kit (Qiagen, 74136). .. RNA samples were reverse transcribed to cDNA using cDNA Synthesis:Article Title: Impaired cortical development and translational control in a missense mouse model of DDX3X syndrome. Article Snippet: Cells were sorted into TRIzol LS Reagent (Invitrogen, 10296028), and RNA was extracted per the manufacturer’s protocol using either TRIzol LS reagent or the Zymo Directzol RNA Miniprep kit (Zymo Research, R2050). .. HEK-293T immortalized cells (ATCC, CRL-3216) were maintained at 37°C, 5% CO2 in DMEM high-glucose medium (Gibco, 11965092) supplemented with 10% fetal bovine serum (HyClone, SH30088.03HI) and 1% Pen-Strep (Gibco, 15140122). qRT-PCR analysis qRT-PCR analyses of gene expressionwere performed as previously described (Hoye et al., 2022), using Article Title: Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cells. Article Snippet: RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Scientific). .. For cDNA synthesis, 250 ng of total RNA was reverse-transcribed in a 20 μL reaction using 4 μL 5x iScript Reaction Mix and 1 μL SYBR Green Assay:Article Title: Impaired cortical development and translational control in a missense mouse model of DDX3X syndrome. Article Snippet: Cells were sorted into TRIzol LS Reagent (Invitrogen, 10296028), and RNA was extracted per the manufacturer’s protocol using either TRIzol LS reagent or the Zymo Directzol RNA Miniprep kit (Zymo Research, R2050). .. HEK-293T immortalized cells (ATCC, CRL-3216) were maintained at 37°C, 5% CO2 in DMEM high-glucose medium (Gibco, 11965092) supplemented with 10% fetal bovine serum (HyClone, SH30088.03HI) and 1% Pen-Strep (Gibco, 15140122). qRT-PCR analysis qRT-PCR analyses of gene expressionwere performed as previously described (Hoye et al., 2022), using |